KMID : 0624620170500020103
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BMB Reports 2017 Volume.50 No. 2 p.103 ~ p.108
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Heme oxygenase-1 (HO-1)/carbon monoxide (CO) axis suppresses RANKL-induced osteoclastic differentiation by inhibiting redox-sensitive NF-¥êB activation
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Bak Sun-Uk
Kim Su-Ji Hwang Hae-Jun Yun Jung-A Kim Wan-Sung Won Moo-Ho Kim Ji-Yoon Ha Kwon-Soo Kwon Young-Guen Kim Young-Myeong
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Abstract
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Heme oxygenase (HO-1) catalyzes heme to carbon monoxide (CO), biliverdin/bilirubin, and iron and is known to prevent the pathogenesis of several human diseases. We assessed the beneficial effect of heme degradation products on osteoclastogenesis induced by receptor activator of NF-¥êB ligand (RANKL). Treatment of RAW264.7 cells with CORM-2 (a CO donor) and bilirubin, but not with iron, decreased RANKL-induced osteoclastogenesis, with CORM-2 having a more potent anti-osteogenic effect. CORM-2 also inhibited RANKL-induced osteoclastogenesis and osteoclastic resorption activity in marrow-derived macrophages. Treatment with hemin, a HO-1 inducer, strongly inhibited RANKL-induced osteoclastogenesis in wild-type macrophages, but was ineffective in HO-1+/? cells. CORM-2 reduced RANKL-induced NFATc1 expression by inhibiting IKK-dependent NF-¥êB activation and reactive oxygen species production. These results suggest that CO potently inhibits RANKL-induced osteoclastogenesis by inhibiting redox-sensitive NF-¥êB-mediated NFATc1 expression. Our findings indicate that HO-1/CO can act as an antiresorption agent and reduce bone loss by blocking osteoclast differentiation.
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KEYWORD
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HO-1/CO, NF-¥êB, Osteoclastogenesis, RANKL
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